22IT

Crystal structure of LbSCT1 in complex with Spermidine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-27
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979
Spacegroup name	P 43
Unit cell lengths	64.340, 64.340, 113.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.664 - 2.120
R-factor	0.220365195409
Rwork	0.218
R-free	0.25605
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.503
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX (1.12_2829)
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.700	2.180
High resolution limit [Å]	2.120	2.120
Number of reflections	26258	1920
<I/σ(I)>	14.6
Completeness [%]	100.0
Redundancy	13.6
CC(1/2)	0.998	0.626

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291.2	0.1 M Sodium cacodylate pH 5.5, 25% w/v PEG 4000