22HR

Crystal structure of Apo FtsZ from Acinetobacter baumannii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.87313
Spacegroup name	P 1
Unit cell lengths	43.944, 44.826, 85.392
Unit cell angles	76.41, 81.31, 64.45

Refinement procedure

Resolution	39.614 - 3.000
Rwork	0.245
R-free	0.28510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6umk
RMSD bond length	0.004
RMSD bond angle	0.953
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.614	3.180
High resolution limit [Å]	3.000	3.000
Rmerge	0.075	0.239
Number of reflections	10579	1704
<I/σ(I)>	4
Completeness [%]	92.7
Redundancy	1.9
CC(1/2)	0.980	0.880

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M MIB, pH-9.0, 25% PEG 1500