22FX
Cryo-EM structure of mouse heavy-chain apoferritin at 1.24 A on CRYO ARM 200 II
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | JEOL CRYO ARM 200 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 1.5 |
| Energy Filter | In-column Omega Filter |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 1.24 |
| Number of Particle | 652257 |
| Software | cryoSPARC,Coot,SerialEM,Servalcat |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
