22FH
Crystal structure of the reduced state of TRP14 from Schistosoma japonicum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 53.435, 38.616, 56.153 |
| Unit cell angles | 90.00, 90.04, 90.00 |
Refinement procedure
| Resolution | 38.724 - 1.771 |
| Rwork | 0.212 |
| R-free | 0.27630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.616 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.724 | 1.820 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Number of reflections | 22103 | 1553 |
| <I/σ(I)> | 7.9 | |
| Completeness [%] | 98.3 | |
| Redundancy | 6.2 | |
| CC(1/2) | 0.995 | 0.482 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 5mM DTT, 0.2 M lithium sulfate monohydrate, 0.1 M HEPES (pH 5.5), and 25% polyethylene glycol 3350 |
