22AO

Crystal structure of Bacillus cereus GmaR in the apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2021-03-07
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	P 43 21 2
Unit cell lengths	82.596, 82.596, 193.971
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.384 - 2.800
R-factor	0.2097
Rwork	0.207
R-free	0.25610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	model deterimined by MAD phasing
RMSD bond length	0.006
RMSD bond angle	0.846
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.850
High resolution limit [Å]	2.800	2.800
Number of reflections	16959	836
<I/σ(I)>	28.8	4.9
Completeness [%]	97.6	99.1
Redundancy	4.9	5.1
CC(1/2)	0.997	0.941

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	PEG 3350, Tris