22YZ

Crystal structure of RipNE220A(82-474) from Ralstonia solanacearum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97930
Spacegroup name	P 32 2 1
Unit cell lengths	127.106, 127.106, 102.852
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.800
R-factor	0.16573
Rwork	0.164
R-free	0.19802
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.310
Data reduction software	autoPX
Phasing software	PHASER
Refinement software	REFMAC (5.8.0415)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.850
High resolution limit [Å]	1.800	1.800
Number of reflections	88786	6514
<I/σ(I)>	24
Completeness [%]	100.0
Redundancy	19.8
CC(1/2)	1.000	0.730

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287	0.2 M ammonium acetate, 28% (w/v) PEG 3350