22ID

Structure of Chaetomium thermophilum Ufd1 UT3 domain fused with a ubiquitin C-terminal peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97853
Spacegroup name	C 2 2 21
Unit cell lengths	64.349, 67.465, 177.666
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.420 - 2.140
R-factor	0.2549
Rwork	0.251
R-free	0.29780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.573
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (v1.21.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.560	2.260
High resolution limit [Å]	2.140	2.140
Rmerge	0.146
Rmeas	0.159
Rpim	0.062
Number of reflections	21753	3096
<I/σ(I)>	13.5
Completeness [%]	99.9
Redundancy	12.3
CC(1/2)	0.998	0.921

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1 M Tris pH 8.0, 28% w/v Polyethylene glycol 4,000