229L
GENERATING LIGAND BINDING SITES IN T4 LYSOZYME USING DEFICIENCY-CREATING SUBSTITUTIONS
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1993-07-20 |
| Detector | XUONG-HAMLIN MULTIWIRE |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.600, 60.600, 97.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| Rwork | 0.160 |
| Structure solution method | DIFFERENCE FOURIER |
| Starting model (for MR) | CYS-FREE WILD TYPE LYSOZYME |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.000 * |
| Data reduction software | SDMS (DETECTOR SYSTEM (NIELSEN)) |
| Data scaling software | SDMS (DETECTOR SYSTEM (NIELSEN)) |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.033 | 0.200 |
| Number of reflections | 18659 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 92.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 6.7 * | 277 | Remington, S.J., (1978). J. Mol. Biol., 118, 81. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 20 (mg/ml) | |
| 2 | 1 | 1 | 0.55 (M) | ||
| 3 | 1 | 1 | mercaptoethanol | 14 (mM) | |
| 4 | 1 | 1 | 1 (mM) | ||
| 5 | 1 | 1 | sodium phospahte | 0.01 (M) | |
| 6 | 1 | 1 | 2.2 (M) | ||
| 7 | 1 | 1 | 1.8 (M) |
