221L
THE ENERGETIC COST AND THE STRUCTURAL CONSEQUENCES OF BURYING A HYDROXYL GROUP WITHIN THE CORE OF A PROTEIN DETERMINED FROM ALA TO SER AND VAL TO THR SUBSTITUTIONS IN T4 LYSOZYME
Experimental procedure
Spacegroup name | P 32 2 1 |
Unit cell lengths | 61.100, 61.100, 97.000 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | ? - 1.700 |
R-factor | 0.158 |
RMSD bond length | 0.017 |
RMSD bond angle | 2.100 |
Refinement software | TNT |
Data quality characteristics
Overall | |
High resolution limit [Å] | 1.700 * |
Rmerge | 0.057 * |
Number of reflections | 15924 * |
Completeness [%] | 67.0 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | potassium phosphate | 1.1 (M) | |
2 | 1 | 1 | sodium phosphate | 1.1 (M) | |
3 | 1 | 1 | 0.15 (M) | ||
4 | 1 | 1 | mercaptoethanol | 1.4 (mM) | |
5 | 1 | 1 | protain | 3.5 (mg/ml) |