21LS

Crystal structure of Horse spleen L-ferritin mutant (L24G/S27G/E56A/R59G/E60A/E63A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E DW
Temperature [K]	93
Detector technology	PIXEL
Collection date	2023-11-13
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.54184
Spacegroup name	F 4 3 2
Unit cell lengths	181.566, 181.566, 181.566
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.700 - 1.570
R-factor	0.2038
Rwork	0.203
R-free	0.22190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.849
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.700	1.600
High resolution limit [Å]	1.570	1.570
Rmerge	0.060	0.897
Rmeas	0.063	0.965
Rpim	0.018	0.352
Number of reflections	36283	1766
<I/σ(I)>	25.7	2
Completeness [%]	100.0	100
Redundancy	10.7	7.4
CC(1/2)	1.000	0.862

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	Ammonium sulfate, Cadmium sulfate