21LR

Crystal structure of Horse spleen L-ferritin mutant (L24G/S27G/E56W/R59G/E60W/E63W)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E DW
Temperature [K]	93
Detector technology	PIXEL
Collection date	2023-11-13
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.54184
Spacegroup name	F 4 3 2
Unit cell lengths	181.144, 181.144, 181.144
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.130 - 1.500
R-factor	0.1897
Rwork	0.189
R-free	0.20660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.946
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.130	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.046	0.368
Rmeas	0.048	0.394
Rpim	0.013	0.138
Number of reflections	41188	2012
<I/σ(I)>	36.5	5.1
Completeness [%]	100.0	100
Redundancy	11.8	7.8
CC(1/2)	1.000	0.954

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	Ammonium sulfate, Cadmium sulfate