21LQ

Crystal structure of Horse spleen L-ferritin mutant (L24G/S27G/E56Y/R59G/E60Y/E63Y)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E DW
Temperature [K]	93
Detector technology	PIXEL
Collection date	2023-11-13
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.54184
Spacegroup name	F 4 3 2
Unit cell lengths	180.878, 180.878, 180.878
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.100 - 1.760
R-factor	0.2179
Rwork	0.217
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.011
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.100	1.800
High resolution limit [Å]	1.760	1.760
Rmerge	0.084	0.856
Rmeas	0.088	0.911
Rpim	0.024	0.305
Number of reflections	25653	1422
<I/σ(I)>	16.7	2
Completeness [%]	99.9	100
Redundancy	11.4	8.4
CC(1/2)	0.999	0.912

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	Ammonium sulfate, Cadmium sulfate