21LP

Crystal structure of Horse spleen L-ferritin mutant (L24G/S27G/E56F/R59G/E60F/E63F)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E DW
Temperature [K]	93
Detector technology	PIXEL
Collection date	2022-12-25
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.54184
Spacegroup name	F 4 3 2
Unit cell lengths	181.150, 181.150, 181.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.130 - 1.570
R-factor	0.1948
Rwork	0.194
R-free	0.21330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.994
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.130	1.600
High resolution limit [Å]	1.570	1.570
Rmerge	0.071	0.805
Rmeas	0.074	0.866
Rpim	0.022	0.312
Number of reflections	36031	1742
<I/σ(I)>	23.4	2.3
Completeness [%]	100.0	100
Redundancy	10.6	7.4
CC(1/2)	0.999	0.831

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	Ammonium sulfate, Cadmium sulfate