21LE

Crystal strucrue of HuHF-C2-CAR complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-12-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	F 4 3 2
Unit cell lengths	182.708, 182.708, 182.708
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.450 - 2.000
R-factor	0.1534
Rwork	0.150
R-free	0.18700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	0.981
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	2.000	5.430	2.000
Rmerge	0.186	0.150	0.305
Rmeas	0.188	0.153	0.309
Rpim	0.032	0.028	0.049
Total number of observations	663957
Number of reflections	18180	1052	884
<I/σ(I)>	6.2
Completeness [%]	100.0	99.9	100
Redundancy	36.5	29.5	39.6
CC(1/2)	0.994	0.995	0.988

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	Bicine, MgCl2