21KW

Crystal strucrue of HuHF-C2-SEM complex

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-12-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	F 4 3 2
Unit cell lengths	182.150, 182.150, 182.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.460 - 2.000
R-factor	0.1695
Rwork	0.166
R-free	0.20010
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.900
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	2.000	5.430	2.000
Rmerge	0.123	0.066	0.698
Rmeas	0.124	0.067	0.707
Rpim	0.020	0.012	0.115
Total number of observations	672631
Number of reflections	18110	1047	885
<I/σ(I)>	6
Completeness [%]	100.0	100	100
Redundancy	37.1	31.8	36.9
CC(1/2)	0.995	0.999	0.957

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	Bicine, MgCl2