21KG
Faecalibacterium duncaniae branching enzyme FdBE2 maltohexaose complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-12 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 165.395, 73.476, 56.971 |
| Unit cell angles | 90.00, 91.84, 90.00 |
Refinement procedure
| Resolution | 47.610 - 1.600 |
| R-factor | 0.14942 |
| Rwork | 0.148 |
| R-free | 0.16749 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.297 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.600 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.023 | 0.265 |
| Rpim | 0.015 | 0.192 |
| Number of reflections | 88773 | 3850 |
| <I/σ(I)> | 19.7 | 2.8 |
| Completeness [%] | 98.8 | 86.5 |
| Redundancy | 3.3 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PEG4000, PEG3350, ammonium sulfate, Tris-HCl pH8.5 |
