21KE

E coli CNF1 D5 domain in complex with BCAM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97923
Spacegroup name	P 2 21 21
Unit cell lengths	105.446, 110.319, 175.093
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.880 - 2.900
R-factor	0.2257
Rwork	0.224
R-free	0.26710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.134
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.880	2.930
High resolution limit [Å]	2.900	2.900
Rmerge	0.149
Number of reflections	86741	2675
<I/σ(I)>	10.2
Completeness [%]	99.2	97.8
Redundancy	6.6
CC(1/2)	0.997	0.881

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	PEG 6000