21IP

The MIDN Catch-IRF4 (A8C) fusion protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	193
Detector technology	PIXEL
Collection date	2025-12-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 65 2 2
Unit cell lengths	88.397, 88.397, 72.692
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.200 - 3.300
R-factor	0.2524
Rwork	0.248
R-free	0.29090
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.103
Data reduction software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.200	3.560
High resolution limit [Å]	3.300	3.300
Number of reflections	2787	546
<I/σ(I)>	2.9
Completeness [%]	99.8
Redundancy	9.4
CC(1/2)	0.997	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	4% (v/v) tacsimate (pH 4.0) and 12% (w/v) PEG 3,350