21IK

The MIDN Catch-IRF4 (V2T) fusion protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	193
Detector technology	PIXEL
Collection date	2025-12-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 65 2 2
Unit cell lengths	88.289, 88.289, 71.777
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.740 - 2.990
R-factor	0.2383
Rwork	0.234
R-free	0.28060
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.996
Data reduction software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.140	3.170
High resolution limit [Å]	2.990	2.990
Number of reflections	3673	571
<I/σ(I)>	3.3
Completeness [%]	100.0
Redundancy	14.6
CC(1/2)	0.992	0.876

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	4% (v/v) tacsimate (pH 4.0) and 20% (w/v) polyethylene glycol (PEG) 3,350.