21IE

The MIDN Catch-IRF4 (A8S) fusion protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	193
Detector technology	PIXEL
Collection date	2025-12-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 65 2 2
Unit cell lengths	88.617, 88.617, 72.117
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.310 - 3.240
R-factor	0.2792
Rwork	0.278
R-free	0.29400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.685
Data reduction software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.310	3.500
High resolution limit [Å]	3.240	3.240
Number of reflections	2954	580
<I/σ(I)>	3.1
Completeness [%]	100.0
Redundancy	14.7
CC(1/2)	0.999	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	4%(v/v) tacsimate (pH 4.0) and 12%(w/v) PEG 3,350