21FO
CYMAL-7-bound MexB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-13 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 207.041, 168.871, 172.210 |
| Unit cell angles | 90.00, 102.23, 90.00 |
Refinement procedure
| Resolution | 40.550 - 2.300 |
| R-factor | 0.2222 |
| Rwork | 0.221 |
| R-free | 0.25520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.964 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419+SVN) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.550 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 251734 | 7726 |
| <I/σ(I)> | 24.1 | |
| Completeness [%] | 100.0 | |
| Redundancy | 4 | |
| CC(1/2) | 0.999 | 0.406 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M HEPES pH8.0, 0.2 M KCl, 8% PEG4000 |
