21ED

Crystal structure of human PHF20L1(85-142)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979183
Spacegroup name	P 43 21 2
Unit cell lengths	55.849, 55.849, 93.168
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.360 - 2.310
Rwork	0.275
R-free	0.28780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	2.058
Data reduction software	DIALS
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.20.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.900	2.390
High resolution limit [Å]	2.310	2.310
Number of reflections	6920	652
<I/σ(I)>	13.5
Completeness [%]	100.0
Redundancy	1.8
CC(1/2)	0.999	0.974

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		289	0.2 M Magnesium formate dihydrate