21AP

Magnaporthe oryzae GH43F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-26
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.97854
Spacegroup name	P 41 21 2
Unit cell lengths	80.030, 80.030, 379.702
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	78.310 - 2.100
R-factor	0.1799
Rwork	0.178
R-free	0.21520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.673
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (v1.20.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.310	2.175
High resolution limit [Å]	2.100	2.100
Rmerge	0.027
Rmeas	0.038
Rpim	0.027
Number of reflections	73577	7211
<I/σ(I)>	16.58
Completeness [%]	99.9
Redundancy	10.6
CC(1/2)	0.999	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	100 mM Tris pH 8.5, 200 mM MgCl2, 20%(w/v) PEG8000