217D
CRYSTAL STRUCTURES OF THE B-FORM DNA-RNA CHIMER (5'-D(*IP*)-R(*CP*)-D(*IP*)-R(*CP*)-D(*IP*CP*IP*C)-3') COMPLEXED WITH DISTAMYCIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Detector technology | AREA DETECTOR |
| Detector | SIEMENS |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 27.930, 27.930, 57.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.700 |
| R-factor | 0.159 |
| Rwork | 0.159 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 3.400 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.700 |
| Total number of observations | 15870 * |
| Number of reflections | 2794 |
| Completeness [%] | 98.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | pH 7.00, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | MGCL2 | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 1 (mM) | |
| 2 | 1 | drop | sodium cacodylate | 20 (mM) | |
| 3 | 1 | drop | distamycin A hydrochloride | 1 (mM) | |
| 4 | 1 | drop | magnesium chloride | 20 (mM) | |
| 5 | 1 | drop | MPD | 2.5 (%(v/v)) | |
| 6 | 1 | reservoir | 60 (%) |
