213D
CRYSTAL STRUCTURE OF THE A-DNA DECAMER D(CCIGGCCM5CGG) AT 1.6 ANGSTROMS SHOWING THE UNEXPECTED WOBBLE I.M5C BASE PAIR
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 290 |
| Detector technology | DIFFRACTOMETER |
| Detector | SIEMENS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 25.020, 44.950, 47.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.600 |
| R-factor | 0.163 |
| Rwork | 0.163 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 31.900 * |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.600 |
| Rmerge | 0.021 * |
| Total number of observations | 21000 * |
| Number of reflections | 6143 |
| Completeness [%] | 86.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | pH 7.00, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | SPERMINE_HCL | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 2 (mM) | |
| 2 | 1 | drop | sodium cacodylate | 40 (mM) | |
| 3 | 1 | drop | spermine tetrachloride | 5 (mM) | |
| 4 | 1 | reservoir | MPD | 50 (%(v/v)) |
