20GS
GLUTATHIONE S-TRANSFERASE P1-1 COMPLEXED WITH CIBACRON BLUE
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-05-31 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 78.176, 90.690, 68.755 |
Unit cell angles | 90.00, 97.73, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.450 |
R-factor | 0.229 |
Rwork | 0.229 |
R-free | 0.28000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5gss |
RMSD bond length | 0.009 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.851) |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.540 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.118 | 0.391 |
Number of reflections | 16729 | |
<I/σ(I)> | 9.2 | 2.7 |
Completeness [%] | 95.8 | 93.9 |
Redundancy | 2.97 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | vapor diffusion - hanging drop - streak seeding | 6 | CRYSTALLISATION PERFORMED USING THE HANGING DROP TECHNIQUE. 2 MICROL OF PROTEIN SOLUTION (8MG/ML) IN 10MM PHOSPHATE BUFFER PH7.0, 1MM EDTA AND 2MM BETA-MERCAPTOETHANOL WAS ADDED TO 2 MICROL OF RESERVOIR SOLUTION. THE RESERVOIR CONTAINED 20-25% AMMONIUM SULFATE, 30-60 MM DITHIOTHREITOL AND 100MM MORPHOLINOETHANESULFONIC ACID BUFFER PH5.4. DROPS WERE STREAK SEEDED AFTER 1 DAY USING A CAT'S WHISKER FROM CRYSTALS GROWN UNDER SIMILAR CONDITIONS. SOLID CIBACRON BLUE WAS SEEDED INTO THE DROP. SIX WEEKS LATER THE CRYSTALS WERE SUBJECTED TO X-RAY ANALYSIS., pH 6.0, vapor diffusion - hanging drop - streak seeding |