209L

PROTEIN STRUCTURE PLASTICITY EXEMPLIFIED BY INSERTION AND DELETION MUTANTS IN T4 LYSOZYME

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1993-04-30
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 62 2 2
Unit cell lengths	95.850, 95.850, 117.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	21.370 - 2.700
R-factor	0.179 *
Rwork	0.179
RMSD bond length	0.015
RMSD bond angle	2.850
Data reduction software	UCSD
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	21.370
High resolution limit [Å]	2.700
Rmerge	0.069
Number of reflections	7329
Completeness [%]	79.5
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG8000	18 (%)
2	1	1	HEPES	0.1 (M)
3	1	1	beta-mercaptoethanol	50 (mM)