209D
Structural, physical and biological characteristics of RNA:DNA binding agent N8-actinomycin D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Detector technology | IMAGE PLATE |
| Detector | MACSCIENCE DIP100S |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 62.300, 62.300, 42.970 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 7.000 - 3.000 |
| R-factor | 0.173 |
| Rwork | 0.173 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 25.500 * |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 7.000 |
| High resolution limit [Å] | 3.000 |
| Rmerge | 0.072 * |
| Number of reflections | 1845 |
| Completeness [%] | 95.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 26 * | PH 7.00, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 299.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | CACODYLATE | ||
| 5 | 1 | 1 | SPERMINE_HCL | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | oligonucleotide | 2.0 (mM) | |
| 2 | 1 | drop | 10 (mM) | ||
| 3 | 1 | drop | cacodylate | 20 (mM) | |
| 4 | 1 | drop | spermine tetrachloride | 2 (mM) | |
| 5 | 1 | drop | N8AMD | 1.5 (mM) | |
| 6 | 1 | drop | MPD | 7.5 (%(v/v)) | |
| 7 | 1 | reservoir | 15 (%) |
