209D
Structural, physical and biological characteristics of RNA:DNA binding agent N8-actinomycin D
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Detector technology | IMAGE PLATE |
Detector | MACSCIENCE DIP100S |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 62.300, 62.300, 42.970 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 7.000 - 3.000 |
R-factor | 0.173 |
Rwork | 0.173 |
RMSD bond length | 0.018 |
RMSD bond angle | 25.500 * |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 7.000 |
High resolution limit [Å] | 3.000 |
Rmerge | 0.072 * |
Number of reflections | 1845 |
Completeness [%] | 95.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 26 * | PH 7.00, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 299.00K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | WATER | ||
2 | 1 | 1 | MPD | ||
3 | 1 | 1 | MGCL2 | ||
4 | 1 | 1 | CACODYLATE | ||
5 | 1 | 1 | SPERMINE_HCL | ||
6 | 1 | 2 | WATER | ||
7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | oligonucleotide | 2.0 (mM) | |
2 | 1 | drop | 10 (mM) | ||
3 | 1 | drop | cacodylate | 20 (mM) | |
4 | 1 | drop | spermine tetrachloride | 2 (mM) | |
5 | 1 | drop | N8AMD | 1.5 (mM) | |
6 | 1 | drop | MPD | 7.5 (%(v/v)) | |
7 | 1 | reservoir | 15 (%) |