205L
HOW AMINO-ACID INSERTIONS ARE ALLOWED IN AN ALPHA-HELIX OF T4 LYSOZYME
Experimental procedure
Spacegroup name | P 32 2 1 |
Unit cell lengths | 60.900, 60.900, 96.100 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | ? - 2.100 |
R-factor | 0.169 |
RMSD bond length | 0.017 |
RMSD bond angle | 2.500 |
Refinement software | TNT |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method * | 6.7 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 20 (mg/ml) | |
2 | 1 | 1 | 0.55 (M) | ||
3 | 1 | 1 | mercaptoethanol | 14 (mM) | |
4 | 1 | 1 | 1 (mM) | ||
5 | 1 | 1 | sodium phospahte | 0.01 (M) | |
6 | 1 | 1 | 2.2 (M) | ||
7 | 1 | 1 | 1.8 (M) |