205D
STRUCTURE OF AN RNA DOUBLE HELIX INCLUDING URACIL-URACIL BASE PAIRS IN AN INTERNAL LOOP
Experimental procedure
Source type | SYNCHROTRON |
Source details | SSRL |
Synchrotron site | SSRL |
Detector technology | IMAGE PLATE |
Collection date | 1993-07-01 |
Detector | MARRESEARCH |
Spacegroup name | P 41 |
Unit cell lengths | 32.440, 32.440, 85.480 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.640 |
R-factor | 0.197 |
Rwork | 0.197 |
RMSD bond length | 0.028 |
RMSD bond angle | 3.600 |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.640 |
Rmerge | 0.108 |
Number of reflections | 2415 |
Completeness [%] | 90.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | pH 5.60, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | WATER | ||
2 | 1 | 1 | PEG | ||
3 | 1 | 1 | EDTA | ||
4 | 1 | 1 | NH4 ACETATE | ||
5 | 1 | 1 | NA CITRATE | ||
6 | 1 | 1 | SPERMINE_HCL | ||
7 | 1 | 2 | WATER | ||
8 | 1 | 2 | PEG |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | RNA | 2 (mM) | |
2 | 1 | drop | EDTA | 0.1 (mM) | |
3 | 1 | reservoir | ammonium acetate | 0.2 (M) | |
4 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.6 |
5 | 1 | reservoir | PEG4000 | 30 (%) | |
6 | 1 | reservoir | spermine tetrahydrochloride | 8 (mM) |