205D
STRUCTURE OF AN RNA DOUBLE HELIX INCLUDING URACIL-URACIL BASE PAIRS IN AN INTERNAL LOOP
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL |
| Synchrotron site | SSRL |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-07-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 |
| Unit cell lengths | 32.440, 32.440, 85.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.640 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| RMSD bond length | 0.028 |
| RMSD bond angle | 3.600 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.640 |
| Rmerge | 0.108 |
| Number of reflections | 2415 |
| Completeness [%] | 90.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | pH 5.60, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | PEG | ||
| 3 | 1 | 1 | EDTA | ||
| 4 | 1 | 1 | NH4 ACETATE | ||
| 5 | 1 | 1 | NA CITRATE | ||
| 6 | 1 | 1 | SPERMINE_HCL | ||
| 7 | 1 | 2 | WATER | ||
| 8 | 1 | 2 | PEG |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | RNA | 2 (mM) | |
| 2 | 1 | drop | EDTA | 0.1 (mM) | |
| 3 | 1 | reservoir | ammonium acetate | 0.2 (M) | |
| 4 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.6 |
| 5 | 1 | reservoir | PEG4000 | 30 (%) | |
| 6 | 1 | reservoir | spermine tetrahydrochloride | 8 (mM) |
