1ZZY
Crystal Structure of Thioredoxin Mutant L7V
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2005-01-07 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 33.658, 36.597, 42.836 |
| Unit cell angles | 78.43, 67.38, 88.39 |
Refinement procedure
| Resolution | 38.690 - 2.500 |
| Rwork | 0.223 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | A:2trx.pdb |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.260 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 5671 | |
| <I/σ(I)> | 6.57 | 2.82 |
| Completeness [%] | 88.7 | 85.2 |
| Redundancy | 1.46 | 1.38 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | COUNTER-DIFFUSION | 3.8 | 295 | 10 mM AcNa pH 3.8, 25% (v/v) EtOH, 10 mM Ac2Cu, Counter-diffusion, temperature 295K |
