1ZZN
Crystal structure of a group I intron/two exon complex that includes all catalytic metal ion ligands.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 108.466, 108.466, 246.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.040 - 3.370 |
| R-factor | 0.269 |
| Rwork | 0.269 |
| R-free | 0.30700 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1u6b |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.490 |
| High resolution limit [Å] | 3.370 | 3.370 |
| Rmerge | 0.072 | |
| Number of reflections | 20658 | |
| <I/σ(I)> | 18.2 | 1.1 |
| Completeness [%] | 95.9 | 71.1 |
| Redundancy | 5.5 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | MPD, sodium cacodylate, magnesium acetate, potassium chloride, cobalt heximine, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
