1ZZM
Crystal structure of YJJV, TATD Homolog from Escherichia coli k12, at 1.8 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.186, 45.007, 57.824 |
| Unit cell angles | 90.00, 100.75, 90.00 |
Refinement procedure
| Resolution | 19.870 - 1.800 |
| R-factor | 0.171 |
| Rwork | 0.171 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j6o |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.900 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.100 | 0.500 |
| Number of reflections | 22291 | |
| <I/σ(I)> | 11.5 | 3 |
| Completeness [%] | 99.4 | 99.1 |
| Redundancy | 3.3 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 279 | 0.01M zinc sulfate, 0.1M MES, 25% PEG MME 550, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 279K |
