1ZZI
Crystal Structure Analysis of the third KH domain of hnRNP K in complex with ssDNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.94 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 52.590, 52.590, 104.891 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.19685 |
| Rwork | 0.195 |
| R-free | 0.24041 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.894 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.300 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.062 | 0.410 |
| Number of reflections | 15859 | |
| <I/σ(I)> | 23.1 | 3.9 |
| Completeness [%] | 98.0 | 87.6 |
| Redundancy | 6.9 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | PEG 4000, TRIS HCl, MAGNESIUM CHLORIDE, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MgCl2 | ||
| 2 | 1 | 1 | PEG 4000 | ||
| 3 | 1 | 1 | TRIS HCl | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | MgCl2 | ||
| 6 | 1 | 2 | PEG 4000 | ||
| 7 | 1 | 3 | H2O |
