1ZZ8
Crystal Structure of FeII HppE in Complex with Substrate Form 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 111.649, 111.649, 152.069 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.300 |
R-factor | 0.225 |
Rwork | 0.225 |
R-free | 0.25200 |
Structure solution method | RIGID BODY REFINEMENT |
Starting model (for MR) | 1zz9 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 42988 | |
<I/σ(I)> | 20.8 | 6.8 |
Completeness [%] | 99.2 | 99.3 |
Redundancy | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | pH 8.5 |