1ZYV
Crystal Structure Of Mutant K8DP9SR58KV59G Of Scorpion alpha-Like Neurotoxin Bmk M1 From Buthus Martensii Karsch
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2004-05-11 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 47.361, 43.874, 25.516 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.460 - 1.500 |
| R-factor | 0.17 |
| Rwork | 0.170 |
| R-free | 0.19800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1SN1.pdb |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.500 | 1.590 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.041 | 0.098 |
| Number of reflections | 8962 | |
| <I/σ(I)> | 10 | 7.1 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 30% (w/v) PEG4000, 0.1M Tris-HCl pH 8.5, 0.2M lithium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
