1ZYT
Crystal structure of spin labeled T4 Lysozyme (A82R1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-D |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-02-23 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 60.293, 60.293, 96.079 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 52.200 - 1.700 |
R-factor | 0.18709 |
Rwork | 0.186 |
R-free | 0.20564 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1c6t |
RMSD bond length | 0.009 |
RMSD bond angle | 1.286 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 22843 | |
<I/σ(I)> | 41.2 | 5.98 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 9.3 | 8.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 293 | potassium phosphate, sodium phospahte, sodium choloride, sodium azide, oxidized beta-mercaptoehtanol , pH 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |