1ZYP
Synchrotron reduced form of the N-terminal domain of Salmonella typhimurium AhpF
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Wavelength(s) | 1.01 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 107.943, 107.943, 85.269 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.270 - 2.400 |
Rwork | 0.205 |
R-free | 0.26090 |
Structure solution method | MOLECULAR REPLACEMENT |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.270 | 2.550 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 19094 | |
Completeness [%] | 94.3 | 86.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 298 | 30-35% PEG 4000, 0.2M ammonium acetate, 0.1M Tris , pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |