1ZYL
Crystal Structure of Hypothetical Protein YihE from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-14 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 90.974, 90.974, 110.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.000 - 2.800 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.27900 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.350 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.131 | 0.539 |
| Number of reflections | 20263 | |
| <I/σ(I)> | 27.4 | 5.1 |
| Completeness [%] | 98.2 | 100 |
| Redundancy | 14.4 | 13.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 298 | Lithium Sulfate, Tris-Hcl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 8.50 |
