1ZYK
Anthranilate Phosphoribosyltransferase in complex with PRPP, anthranilate and magnesium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-02-11 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.933 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 92.556, 66.099, 117.069 |
Unit cell angles | 90.00, 107.76, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.400 |
Rwork | 0.207 |
R-free | 0.26900 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1o17 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.329 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.500 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 52279 | |
<I/σ(I)> | 15.33 | 5.45 |
Completeness [%] | 96.9 | 87.1 |
Redundancy | 3.76 | 3.03 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG 1500, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |