1ZY9
Crystal structure of Alpha-galactosidase (EC 3.2.1.22) (Melibiase) (tm1192) from Thermotoga maritima at 2.34 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-05-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979413, 0.918370, 0.979170 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 121.729, 160.533, 88.258 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.504 - 2.340 |
Rwork | 0.162 |
R-free | 0.21300 |
Structure solution method | MAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.550 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.880 | 42.880 | 2.470 |
High resolution limit [Å] | 2.340 | 7.400 | 2.340 |
Rmerge | 0.129 | 0.042 | 0.603 |
Number of reflections | 35967 | ||
<I/σ(I)> | 5.7 | 13.6 | 1.3 |
Completeness [%] | 98.0 | ||
Redundancy | 3.7 | 3.6 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP, NANODROP | 8.3 | 273 | 0.2M K3Cirtrate, 20.0% PEG-3350, No Buffer, pH 8.3, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 273K |