1ZXT
Crystal Structure of A Viral Chemokine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.452, 40.636, 55.092 |
| Unit cell angles | 83.54, 89.68, 79.19 |
Refinement procedure
| Resolution | 40.000 - 1.700 |
| R-factor | 0.194 |
| Rwork | 0.189 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cm9 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.957 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.044 | 0.650 |
| Number of reflections | 28953 | |
| <I/σ(I)> | 14.8 | 1.4 |
| Completeness [%] | 94.7 | 89.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | vapor diffusion, sitting drops | 1:1 10mg/ml protein: mother liquor (1.2M NaCl,0.1M NaOAc pH5.5, 22oC), vapor diffusion, sitting drops |
