1ZXO
X-ray Crystal Structure of Protein Q8A1P1 from Bacteroides thetaiotaomicron. Northeast Structural Genomics Consortium Target BtR25.
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-03-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97908,0.97932,0.96797 |
Spacegroup name | H 3 |
Unit cell lengths | 158.154, 158.154, 275.531 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.560 - 3.200 |
R-factor | 0.241 |
Rwork | 0.241 |
R-free | 0.29700 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.083 | 0.549 |
<I/σ(I)> | 24.64 | 3.2 |
Completeness [%] | 99.7 | 99.6 |
Redundancy | 4.79 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 277 | 8% PEG8K, 0.1M NaHepes, 8% Ethyl-Glycol , pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K |