1ZXK
Crystal Structure of Cadherin8 EC1 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2003-12-18 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 46.196, 39.934, 66.439 |
Unit cell angles | 90.00, 110.68, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.25358 |
Rwork | 0.252 |
R-free | 0.28336 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zvn |
RMSD bond length | 0.012 |
RMSD bond angle | 1.439 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.060 |
High resolution limit [Å] | 1.990 | 1.990 |
Rmerge | 0.078 | 0.201 |
Number of reflections | 15229 | |
<I/σ(I)> | 10.4 | 3.9 |
Completeness [%] | 99.0 | 97.3 |
Redundancy | 2.8 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 22% PEG 4000, 0.1M HEPES pH7, 0.1M NaAcetate, VAPOR DIFFUSION, HANGING DROP, temperature 277K |