1ZX3
Structure of NE0241 Protein of Unknown Function from Nitrosomonas europaea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-03 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97924 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 77.329, 77.329, 84.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.500 |
| R-factor | 0.21895 |
| Rwork | 0.215 |
| R-free | 0.26055 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.789 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.570 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.076 | 0.788 |
| Number of reflections | 4689 | |
| <I/σ(I)> | 43.9 | 3.29 |
| Completeness [%] | 99.7 | 98.6 |
| Redundancy | 13.3 | 11.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 294 | 0.1 M Bis-Tris, 1.4 M sodium citrate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
