1ZWY
Crystal structure of protein VC0702 from Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-29 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97970 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.399, 105.347, 75.272 |
| Unit cell angles | 90.00, 104.57, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.18272 |
| Rwork | 0.180 |
| R-free | 0.22552 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.481 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.066 | 0.684 |
| Number of reflections | 63342 | |
| <I/σ(I)> | 21.15 | 1.84 |
| Completeness [%] | 98.4 | 95.2 |
| Redundancy | 6.2 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | PEG 3000, HEPES, NaCl, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 395K |
