1ZWX

Crystal Structure of SmcL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.1
Synchrotron site	SRS
Beamline	PX14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-01-05
Detector	ADSC QUANTUM 4
Wavelength(s)	1.4880
Spacegroup name	P 31 2 1
Unit cell lengths	66.860, 66.860, 180.848
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.670 - 1.900
R-factor	0.203
Rwork	0.201
R-free	0.23300
Structure solution method	SIRAS
RMSD bond length	0.016
RMSD bond angle	1.433
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE (2.06)
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.680	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.063	0.263
Number of reflections	37481
<I/σ(I)>	18.8	2.7
Completeness [%]	99.4	100
Redundancy	5.7	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	298	PEG 8000, sodium citrate, sodium dihydrogen phosphate, sodium chloride, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
1	VAPOR DIFFUSION, HANGING DROP	4.8	298	PEG 8000, sodium citrate, sodium dihydrogen phosphate, sodium chloride, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K