1ZWL
Structure of WrbA from Pseudomonas aeruginosa in complex with FMN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 42 2 2 |
| Unit cell lengths | 73.540, 73.540, 78.166 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.19858 |
| Rwork | 0.197 |
| R-free | 0.23594 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ydg |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.334 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.110 | 0.277 |
| Number of reflections | 5616 | |
| <I/σ(I)> | 14.8 | 3.93 |
| Completeness [%] | 99.2 | 93.3 |
| Redundancy | 6.2 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 16% PEG 3350, .12M MgCl, .1M Bis pH 6.0, .003M FMN, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
